Regeneration of plants from gynogenetic carrot calluses
Keywords: carrots, experimental haploidy in vitro, non-fertilized ovaries, callus, embryogenesis, regenerant plants
AbstractAim. To optimize the regeneration and adaptation stages of the methods of obtaining dihaploid carrot plants via gynogenesis in the culture in vitro, namely embryogenesis from gynogenetic callus, and to obtain normally developed regenerant plants and regenerant roots. Methods. Common biotechnological methods were applied. The gynogenetic carrot callus, line 345, was obtained from non-fertilized ovaries using the method, invented by the authors. Results. The culture medium, in which the yield of embryos from one gynogenetic callus in- creases 4-fold, and the yield of normal plants – 12-fold, was invented. While growing roots using regenerant plants in unprotected soil the mulching of soil with white agrofi ber ensures the survival of regenerants at the level of 86.5 % which is 16 % higher than the control (without mulching) and the increase in the average weight of roots up to 92.8 g (38.5 g more compared to the control). Conclusions. The regenerating and adaptive stages of obtaining dihaploid regenerant plants from gynogenetic carrot calluses, induced from non-fertilized ovaries in culture in vitro were optimized. The effi cient culture medium for embryogenesis from gynogenetic calluses was elaborated. It was established that soil mulching using white agrofi ber increases the survival of gynoge- netic plants in unprotected soil considerably and ensures the increase in the average weight of roots. It allows refusing from greenhouses or tunnels while growing regenerant roots of gynogenetic origin.
How to Cite
Vitsenia, T., & Sergiyenko, O. (2015). Regeneration of plants from gynogenetic carrot calluses. Agricultural Science and Practice, 2(3), 49-54. https://doi.org/10.15407/agrisp2.03.049
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