Microbial Regulation of the Antagonistic Activity of Lactic Acid Bacteria Strains under the Influence of Transient Bacilli of the Bacillus Genus
Keywords: antagonism, lactic acid bacteria, pathogenic and conditionally pathogenic bacteria, stimulation, metabolites, microbial cell components
AbstractAim. To study the effect of metabolites and components of aerobic spore-forming bacilli cells (MCASB) of the Bacillus genus on the antagonistic activity of lactic acid bacteria. Methods. Lactic acid bacteria, sampled from rectal and oral swabs from calves, were used in the study. Strains of conditionally pathogenic Escherichia coli, 055k59 No. 3912, Staphylococcus aureus No. 906, Proteus mirabilis, No. 3177, obtained from the collection of the city sanitary-epidemiological inspection service, were used as test cultures. MCASB were prepared using autoclaved microbial mass of Bacillus subtilis production strain 44-p via treatment with lysozyme and ultra- sound. The antagonistic activity of lactic acid bacteria was determined by agar blocks method. Results. It was demonstrated that the addition of MCASB to the culture medium stimulates the antagonistic activity of lactic acid bacteria to pathogenic and conditionally pathogenic microorganisms of intestinal bacteria. The highest average stimulation index (2.56) of the antagonistic activity of lactic acid bacteria was found for E. coli 055k59 No. 3912. Conclusions. The results obtained may be used to improve the microbiological foundations of elaborating novel probiotic preparations.
How to Cite
Kravchenko, N. (2015). Microbial Regulation of the Antagonistic Activity of Lactic Acid Bacteria Strains under the Influence of Transient Bacilli of the Bacillus Genus. Agricultural Science and Practice, 2(2), 45-49. https://doi.org/10.15407/agrisp2.02.045
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