Effectiveness of complex inoculation of spring wheat with N[2] -fi xing bacteria Azospirillum brasilense and mold-antagonist Chaetomium cochliodes

  • E. Kopylov -
Keywords: Azospirillum brasilense, nitrogen-fi xing bacteria, spring wheat, endophytes, chlorophyll, Chaeto- mium cochliodes 3250

Abstract

Aim. To study the specifi cities of complex inoculation of spring wheat roots with the bacteria of Azospirillum genus and Chaetomium cochliodes Palliser 3250, and the isolation of bacteria of Azospirillum genus, capable of fi xing atmospheric nitrogen, from the rhizospheric soil, washed-off roots and histoshere. Materials and meth- ods. The phenotypic features of the selected bacteria were identifi ed according to Bergi key. The molecular the polymerase chain reaction and genetic analysis was used for the identifi cation the bacteria. Results. It has been demonstrated that during the introduction into the root system of spring wheat the strain of A. brasilensе 102 actively colonizes rhizospheric soil, root surface and is capable of penetrating into the inner plant tissues. Conclusions. The soil ascomucete of C. cochliodes 3250 promotes better settling down of Azospirillum cells in spring wheat root zone, especially in plant histosphere which induces the increase in the content of chlorophyll a and b in the leaves and yield of the crop.
Published
2014-12-15
How to Cite
Kopylov, E. (2014). Effectiveness of complex inoculation of spring wheat with N[2] -fi xing bacteria Azospirillum brasilense and mold-antagonist Chaetomium cochliodes. Agricultural Science and Practice, 1(3), 57-61. https://doi.org/10.15407/agrisp1.03.057