The usage of cell selection in vitro in the creation of tomato and eggplants breeding lines with resistance to fusarium (Fuzarium oxysporum)

  • T. Ivchenko -
  • S. Kornienko -
  • T. Miroshnichenko -
  • H. Mozgovska -
Keywords: in vitro, Fusarium, selective factor, fungal culture fi ltrate, resistance source, selection

Abstract

Aim. The development of a biotechnological system of assessing and selecting the forms of egg-plant and tomato which are tolerant to fusarium wilt in the culture of the tissues, isolated in vitro. Methods. The studies were car- ried out according to the standard biotechnological methods and using the standard equipment. The high-grade and hybrid seeds of egg-plant and tomato genotypes of F 1 –F 4 breeds with different tolerance to fusarium wilt were used in the studies. The cell selection was carried out in the media with different content of the selective agent (20, 40 and 60 % of the total medium volume). Results. It has been determined that the samples can be reliably differentiated in the selective media with liquid culture fi ltrate on the stages of induction and prolifera- tion of callusogenesis according to their resistance in the fi eld. The effective concentrations of the liquid culture fi ltrate of F. oxysporum in the selective media necessary for the selection of resistant callus clones are 20−30 % for acceptable genotypes and 40–50 % and higher for moderately resistant and resistant samples. According to the selective assessment (2012−2013) of initial breeding lines of egg-plant and tomato and the ones, selected via the cell selection, there were 4 highlighted promising lines of egg-plant and 5 lines of tomato that exceeded the control samples and the initial genotypes in their resistance to fusarium and in fertility. Conclusions. The pos- sibility and effi ciency of the biotechnology of accelerated creation of and express-tests on the breeding lines of egg-plant and tomato resistant to F. oxysporum were substantiated and experimentally proved in order to cut the time necessary for the attainment of resistant initial material.
Published
2014-12-15
How to Cite
Ivchenko, T., Kornienko, S., Miroshnichenko, T., & Mozgovska, H. (2014). The usage of cell selection in vitro in the creation of tomato and eggplants breeding lines with resistance to fusarium (Fuzarium oxysporum). Agricultural Science and Practice, 1(3), 15-21. https://doi.org/10.15407/agrisp1.03.015