National Features of the Nitrate Directive Implementation in Agricultural Practice

  • M.M. Miroshnychenko The National Scientific Center "O. N. Sokolovsky Institute for Soil Science and Agrochemistry Research", 4, Mykhail Semenko Str., Kharkiv, 61024, Ukraine; The National Academy of Agrarian Sciences of Ukraine, 9, Mykhailo Omelianovych-Pavlenko Str., Kyiv, 01010, Ukraine https://orcid.org/0000-0003-2830-5933
  • A.V. Revtie-Uvarova The National Scientific Center "O. N. Sokolovsky Institute for Soil Science and Agrochemistry Research", 4, Mykhail Semenko Str., Kharkiv, 61024, Ukraine https://orcid.org/0000-0002-6838-5440
  • Ye.Yu. Hladkikh The National Scientific Center "O. N. Sokolovsky Institute for Soil Science and Agrochemistry Research", 4, Mykhail Semenko Str., Kharkiv, 61024, Ukraine https://orcid.org/0000-0002-4852-0502
  • T.V. Shevchenko The National Academy of Agrarian Sciences of Ukraine, 9, Mykhailo Omelianovych-Pavlenko Str., Kyiv, 01010, Ukraine https://orcid.org/0000-0001-9488-0325
Keywords: nitrates, mineral nitrogen, migration, temporal dynamics, chornozem

Abstract

Aim. To substantiate the approach towards the identification of zones, vulnerable to nitrate accumulation, based on the data of mineral nitrogen content in soil according to the requirements of the Nitrate Directive. Methods. The study was conducted on arable soils of humus-accumulative genesis on six land plots in the Forest-Steppe of Ukraine with the rate of applying nitrogen-containing fertilizers from 0 to 170 kg/ha. The content of mineral nitrogen (Nmin), nitrates and productive moisture reserves was determined layer-wise down to the depth of 120 cm, in dynamics during the vegetative periods of 2021, 2024, and 2025. Crops: grain corn, winter wheat, spring barley, oil flax. Results. A considerable variability in the content of Nmin and nitrates proper was determined in soil depending on terms, ways, and norms of applying nitrogen-containing fertilizers. The maximal concentrations were observed in the arable soil layer immediately after the application of nitrogen fertilizers, but these parameters gradually decreased with time and depth. In June–July, the highest accumulation of nitrates in deep soil layers was noted when, at the norm of the application of fertilizers of over N100, the content of nitric nitrogen at the depth from 80 to 120 cm was from 15.6 to 35.8 mg/kg. The accumulation of nitrates in deep layers of soil decreases in the course of the vegetation period, and at the time of harvesting, the content of nitric nitrogen at the depth of 80–120 cm did not exceed 10 mg/kg in a year with normal moisturization conditions and 4 mg/kg in a dry year. At the norm of applying fertilizers up to 100 kg/ha, the accumulation of Nmin and nitrates in deep soil layers is minimal. Conclusions. The use of Nmin as an indicator corresponds to the requirements of the Nitrate Directive regarding the identification of zones, vulnerable to the accumulation of nitrates. It is reasonable to take measurements after harvesting the crops. The application of nitrogen-containing fertilizers for the crops of rotations on arable soils of the humus-accumulative genesis in the plains of Ukraine with non-flushing or periodically flushing type of the water regime at the norm, not exceeding 100 kg/ha, does not pose any threat of leaching mineral nitrogen and its nitrate forms from soil and further pollution of the underground waters.

References

Agriculture, forestry and fisheries of Ukraine. Application of mineral and organic fertilizers, pesticides (1990–2023). State Statistics Service of Ukraine. (In Ukrainian). Retrieved from https://www.ukrstat.gov.ua/operativ/menu/menu_u/cg.htm

Council Directive 91/676/EEC of 12 December 1991 concerning the protection of waters against pollution caused by nitrates from agricultural sources: Council of the European Union. Retrieved from https://eurlex.europa.eu/legal-content/EN/ALL/?uri=CELEX%3A31991L0676

D'Haene K, Salomez J, De Neve S, De Waele J, Hofman G (2014) Environmental performance of the nitrogen-fertiliser limits imposed by the EU Nitrates Directive. Agric. Ecosyst. Environ, 192:67–79. https://doi.org/10.1016/j.agee.2014.03.049

Demydenko OV, Velychko VA (2025) Moisturization regime and implementation of chornozem agropotential under climate changes in the central Forest-Steppe. Agricultural Science and Practice, 12(2):66–97. https://doi.org/10.15407/agrisp12.02.066

DSTU 4729:2007 Soil quality. Determination of nitrate and ammonium nitrogen in the modification of the National Scientific Center "Institute for Soil Science and Agrochemistry Research named after O.N. Sokolovsky". Effective from 01.01.2008. Official edition. Kyiv: Derzhspozhyvstandart of Ukraine, 2008. 10 p. (in Ukrainian)

DSTU ISO 11465:2001 (ISO 11465:1993, IDT) Soil quality. Determination of dry matter and moisture content by mass. Gravimetric method. Effective from 01.01.2003. Official edition. Kyiv: Derzhspozhyvstandart of Ukraine, 2002. 5 p. (in Ukrainian)

Engels T, Kuhlmann H (1993) Effect of the rate of N fertilizer on apparent net mineralization of N during and after cultivation of cereal and sugar beet crops. Z. Pflanz. Bodenkd, 156:149–154. https://doi.org/10.1002/jpln.19931560209

Feng GZ, He XL, Coulter JA, Chen YL, Gao Q, Mi GH (2019) Effect of limiting vertical root growth on maize yield and nitrate migration in clay and sandy soils in Northeast China. Soil and Tillage Research, 195:104407. https://doi.org/10.1016/j.still.2019.104407

Fertilizer Consumption — Historical Trends by Country or Region (Fertilizer consumption for N, P2O5 and K2O by country or region). IFASTAT Consumption Database. Retrieved from https://www.ifastat.org/databases/graph/1_1

Galloway JN, Leach AM, Bleeker A, Erisman JW (2013) A chronology of human understanding of the nitrogen cycle. Philosophical Transactions of the Royal Society B, 368: 20130120. https://doi.org/10.1098/rstb.2013.0120

Kant S, Bi YM, Rothstein SJ (2011) Understanding plant response to nitrogen limitation for the improvement of crop nitrogen use efficiency. J Exp Bot., 62(4):1499-509. https://doi.org/10.1093/jxb/erq297

Kundel D, Lori M, Fliessbach A, van Kleunen M, Meyer S, Mader P (2021) Drought Effects on Nitrogen Provisioning in Different Agricultural Systems: Insights Gained and Lessons Learned from a Field Experiment. Nitrogen, 2:1–17. https://doi.org/10.3390/nitrogen2010001

Li Z, Zhang R, Xia S, Wang L, Liu C, Zhang R, Fan Z, Chen F, Liu Y (2019) Interactions between N, P and K fertilizers affect the environment and the yield and quality of satsumas. Global Ecology and Conservation, 19:e00663. https://doi.org/10.1016/j.gecco.2019.e00663

Lori M, Piton G, Symanczik S, Legay N, Brussaard L, Jaenicke S, Nascimento E, Reis F, Sousa JP, Mader P (2020) Compared to conventional, ecological intensive management promotes beneficial proteolytic soil microbial communities for agro-ecosystem functioning under climate change-induced rain regimes. Sci. Rep., 10:1–15. https://doi.org/10.1038/s41598-020-64279-8

Lu T, Wang J, Zhu H, Zhong Z, Wang X, Jia X, Shao M, Wei X (2025) Soil moisture determines effects of climates and soil properties on nitrogen cycling: Examination of arid and humid soils. Journal of Environmental Management, 73:123831. https://doi.org/10.1016/j.jenvman.2024.123831

Methodology for determining zones vulnerable to (accumulation of) nitrates : Order of the Ministry of Environmental Protection and Natural Resources of Ukraine of April 15, 2021 No. 244. (In Ukrainian) Retrieved from https://ips.ligazakon.net/document/RE36398?an=24

Nosko BS (2013) Nitrogen regime of soils and its transformation in agroecosystems. Kharkiv. 130 p. (In Ukrainian)

Osadcha NM, Osadchyi VI, Osypov VV, Biletska SV, Kovalchuk LA, Artemenko VA (2020) Methodology for the nitrate vulnerable zones designation in surface and ground water. Ukr. geogr.zh., 4(112):38–48. (In Ukrainian) https://doi.org/10.15407/ugz2020.04.038

Report from the commission to the council and the European parliament on the implementation of Council Directive 91/676/EEC concerning the protection of waters against pollution caused by nitrates from agricultural sources based on Member State reports for the period 2016–2019. European Commission. Brussels, 11.10.2021 Retrieved from https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=COM%3A2021%3A1000%3AFIN

Romero E, Beusen AHW, Bouwman AF, Einarsson R, Garnier J (2024) Nitrogen in agri-food systems and the environment: Next steps to a sustainable future. Science of The Total Environment, 947:174555. https://doi.org/10.1016/j.scitotenv.2024.174555

Rules for ensuring soil fertility and the use of certain agrochemicals: Order of the Ministry of Agrarian Policy and Food of Ukraine of November 24, 2021 No. 382. (In Ukrainian) Retrieved from https://zakon.rada.gov.ua/laws/show/z0034-22#Text

Rupp H, Tauchnitz N, Meissner R (2021) The Effects of Soil Drying Out and Rewetting on Nitrogen and Carbon Leaching — Results of a Long-Term Lysimeter Experiment. Water, 13(18):2601. https://doi.org/10.3390/w13182601

San Martin W (2020) Global Nitrogen in Sustainable Development: Four Challenges at the Interface of Science and Policy. In: Leal Filho W, Azul A, Brandli L, Lange Salvia A, Wall T (eds) Life on Land. Encyclopedia of the UN Sustainable Development Goals. Springer, Cham 1–16. https://doi.org/10.1007/978-3-319-71065-5_114-1

Sandhu N, Sethi M, Kumar A, Dang D, Singh J, Chhuneja P (2021) Biochemical and genetic approaches improving nitrogen use efficiency in cereal crops: a review. Front. Plant Sci, 12:657629. https://doi.org/10.3389/fpls.2021.657629

Schimel J (2018) Life in Dry Soils: Effects of Drought on Soil Microbial Communities and Processes. Annu. Rev. Ecol. Evol. Syst., 49:409–432. https://doi.org/10.1146/annurev-ecolsys-110617-062614

Sebilo M, Mayer B, Nicolardot B, Pinay G, Mariotti A (2013) Long-term fate of nitrate fertilizer in agricultural soils. The Proceedings of the National Academy of Sciences, 110(45):18185–18189. https://doi.org/10.1073/pnas.1305372110

Soil moisture reserves are decreasing in Ukraine — corn and sunflower harvests are at risk. J. Agronom, 2025. (in Ukrainian) https://www.agronom.com.ua/v-ukrayini-strimko-zmenshuyutsya-zapasy-vology-v-grunti-pid-zagrozoyu-vrozhaj-kukurudzy-j-sonyashnyku/

The improved diagnostics of the level of nitrogen provision in soil using the methods of field and laboratory testing: Scientific and methodological recommendations / A. V. Revtie-Uvarova, O. V. Karatsiuba, V. M. Nikonenko, O. I. Slidenko. Kharkiv: PE Brovyn O. v., 2020. 94 p. ISBN 978-617-7912-82-7 (in Ukrainian)

Werisch S, Burghardt D (2021) Identification of mechanisms controlling nitrogen export from agricultural lysimeters. In Proceedings of the 19th Gumpensteiner Lysimetertagung, Raumber-Gumpenstein, Raumberg, Austria, 13–14 April. Pp. 71–78. ISBN: 978-3-902849-83-0

Wilson L, New S, Daron J, Golding N (2021) Climate Change Impacts for Ukraine. Met Office. 34 p. https://www.metoffice.gov.uk/binaries/content/assets/metofficegovuk/pdf/services/government/met-office_climate-change-impacts-for-ukraine_report_08dec2021_english.pdf

Xu J, Ren C, Zhang X, Wang C, Wang S, Ma B, He Y, Hu L, Liu X, Zhang F, Lu L, Li S, Zhang J, Zhu Y-G, Vitousek P, Gu B (2025) Soil health contributes to variations in crop production and nitrogen use efficiency. Nat Food, 6:597–609. https://doi.org/10.1038/s43016-025-01155-6

Published
2026-04-23
How to Cite
Miroshnychenko, M., Revtie-Uvarova, A., Hladkikh, Y., & Shevchenko, T. (2026). National Features of the Nitrate Directive Implementation in Agricultural Practice. Agricultural Science and Practice, 13(1), 3-19. https://doi.org/https://doi.org/10.15407/agrisp13.01.003