Time series modeling of retained placenta, metritis, and ketosis in Holstein cows and heifers and its association with climate variables in a hot-arid zone

Keywords: dairy cows, heat stress, periparturient diseases, temperature-humidity index, trend analysis

Abstract

Aim. To forecast the monthly percentage of Holstein cows and heifers at a high-input dairy farm experiencing retained placenta (RP), puerperal metritis (PM), and clinical ketosis (CK). Methods. An autoregressive integrated moving average (ARIMA) model was employed to predict future monthly cases of these diseases using time series data. These puerperal diseases were observed on a single dairy farm with 2560 to 3300 milking cows over seven years, from 2014 to 2020. Results. The highest predicted RP incidence in cows was in May (11.3%; 95% CI=6.3-16.4), while the lowest was in November (5.4%; 95% CI=0.5-10.4). For heifers, the peak predicted RP occurrence was in August (20.6%; 95% Cl=11.0-23.1), and the lowest was in December (10.5%; 95% CI=7.9-13.0). The highest projected CK occurrence in cows was in June (3.0%; 95% CI=1.8-4.3) and the lowest was in November (1.1%; 95% CI=-0.1-2.4). For heifers, CK was most likely in May (2.7%; 95% CI=0.9-4.5) and least in December (0.7%; 95% CI=-1.1-2.5). Conclusions. Both cows and heifers showed an increasing trend in RP, PM, and CK during summer months; ARIMA models effectively tracked disease trends throughout the year and can aid in health management decisions for dairy cows.

References

Bagath M, Krishnan G, Devaraj C, Rashamol VP, Pragna P, Lees AM, Sejian V (2019) The impact of heat stress on the immune system in dairy cattle: A review. Res Vet Sci 126:94–102. https://doi.org/10.1016/j.rvsc.2019.08.011

Baumgard LC, Rhoads RP (2013) Effects of heat stress on postabsorptive metabolism and energetics. Annu Rev Anim Biosci 1(1):311–337. https://doi.org/10.1146/annurev-animal-031412-103644

Benzaquen ME, Risco CA, Archbald LF, Melendez P, Thatcher MJ, Thatcher WW (2007) Rectal temperature, calving-related factors, and the incidence of puerperal metritis in postpartum dairy cows. J Dairy Sci 90(6):2804–2814. https://doi.org/10.3168/jds.2006-482

Berge AC, Vertenten G (2014) A field study to determine the prevalence, dairy herd management systems, and fresh cow clinical conditions associated with ketosis in western European dairy herds. J Dairy Sci 97(4):2145–2154. https://doi.org/10.3168/jds.2013-7163

Casarotto LT, Jones HN, Chavatte-Palmer P, Laporta J, Penagaricano F, Ouellet V, Bromfield J, Dahl GE (2025) Late-gestation heat stress alters placental structure and function in multiparous dairy cows. J Dairy Sci 108(1):1125–1137. https://doi.org/10.3168/jds.2024-25529

Chang-Fung-Martel J, Harrison MT, Brown JN, Rawnsley R, Smith AP, Meinke H (2021) Negative relationship between dry matter intake and the temperature-humidity index with increasing heat stress in cattle: A global meta-analysis. Int J Biometeorol 65(12):2099–2109. https://doi.org/10.1007/s00484-021-02167-0

Chauhan SS, Rashamol VP, Bagath M, Sejian V, Dunshea FR (2021) Impacts of heat stress on immune responses and oxidative stress in farm animals and nutritional strategies for amelioration. Int J Biometeorol 65(7):1231–1244. https://doi.org/10.1007/s00484-021-02083-3

Chebel RC (2021) Predicting the risk of retained fetal membranes and metritis in dairy cows according to prepartum hemogram and immune and metabolic status. Prev Vet Med 187:105204. https://doi.org/10.1016/j.prevetmed.2020.105204

Dahl GE, Tao S, Laporta J (2020) Heat stress impacts immune status in cows across the life cycle. Front Vet Sci 7:116. https://doi.org/10.3389/fvets.2020.00116

De Oliveira EB, Cunha F, Daetz R, Figueiredo CC, Chebel RC, Santos JE, Risco CA, Jeong KC, Machado VS, Galvao KN (2020) Using chitosan microparticles to treat metritis in lactating dairy cows. J Dairy Sci 103(8):7377–7391. https://doi.org/10.3168/jds.2019-18028

Funnell BJ, Hilton WM (2016) Management and prevention of dystocia. Vet Clin North Am Food Anim Pract 32(2):511–522. https://doi.org/10.1016/j.cvfa.2016.01.016

Galvao KN (2013) Uterine diseases in dairy cows: understanding the causes and seeking solutions. Anim Reprod 10(3):228–238.

Galvao KN, Bicalho RC, Jeon SJ (2019) Symposium review: The uterine microbiome associated with the development of uterine disease in dairy cows. J Dairy Sci 102(12):11786–11797. https://doi.org/10.3168/jds.2019-17106

Garcia E (1981) Modifications to the Koeppen Climate Classification System to Adapt It to the Conditions of the Mexican Republic. Offset Larios. Mexico. 246 p.

Gernand E, Konig S, Kipp C (2019) Influence of on-farm measurements for heat stress indicators on dairy cow productivity, female fertility, and health. J Dairy Sci 102(7):6660–6671. https://doi.org/10.3168/jds.2018-16011

Ghavi Hossein-Zadeh N, Ardalan M (2011) Cow-specific risk factors for retained placenta, metritis and clinical mastitis in Holstein cows. Vet Res Commun 35(6):345–354. https://doi.org/10.1007/s11259-011-9479-5

Giannone C, Bovo M, Ceccarelli M, Torreggiani D, Tassinari P (2023) Review of the heat stress-induced responses in dairy cattle. Animals 13(22):3451. https://doi.org/10.3390/ani13223451

Gilbert RO (2019) Symposium review: Mechanisms of disruption of fertility by infectious diseases of the reproductive tract. J Dairy Sci 102(5):3754–3765. https://doi.org/10.3168/jds.2018-15602

Giuliodori MJ, Magnasco M, Magnasco RP, Lacau-Mengido IM, de la Sota RL (2017) Purulent vaginal discharge in grazing dairy cows: Risk factors, reproductive performance, and prostaglandin F2α treatment. J Dairy Sci 100(5):3805–3815. https://doi.org/10.3168/jds.2016-11373

Gulinski P (2021) Ketone bodies — causes and effects of their increased presence in cows' body fluids: A review. Vet World 14(6):1492–1503. https://doi.org/10.14202/vetworld.2021.1492-1503

Ha S, Kang S, Jeong M, Han M, Lee J, Chung C, Park J (2023) Characteristics of Holstein cows predisposed to ketosis during the post-partum transition period. Vet Med Sci 9(1):307–314. https://doi.org/10.1002/vms3.1006

Hashem MA, Amer HA (2008) Hormonal and biochemical anomalies in dairy cows affected by retained fetal membranes. Int J Vet Med 185:1517–1519.

Jeon SJ, Galvao KN (2018) An advanced understanding of uterine microbial ecology associated with metritis in dairy cows. Genom Inform 16(4):e21. https://doi.org/10.5808/GI.2018.16.4.e21

Jeong JK, Choi IS, Moon SC, Lee SC, Kang HG, Jung yC, Park SB, Kim IH (2017) Risk factors for ketosis in dairy cows and associations with some blood metabolite concentrations. J Vet Clin 34(4):255–260. https://doi.org/10.17555/jvc.2017.08.34.4.255

Jere S, Moyo E (2016) Modelling epidemiological data using Box-Jenkins procedure. Open J Stat 6(2):295–302. https://doi.org/10.4236/ojs.2016.62025

Kamel ER, Ahmed HA, Hassan FM (2022) The effect of retained placenta on the reproductive performance and its economic losses in a Holstein dairy herd. Iraqi J Vet Sci 36(2):359–365. https://doi.org/10.33899/ijvs.2021.130287.1791

Koch F, Thom U, Albrecht E, Weikard R, Nolte W, Kuhla B, Kuehn C (2019) Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine. Proc Natl Acad Sci USA 116(21):10333–10338. https://doi.org/10.1073/pnas.1820130116

Lacetera N (2019) Impact of climate change on animal health and welfare. Anim Front 9(1):26–31. https://doi.org/10.1093/af/vfy030

Li y, Wen C, yang y, Zhao Z, Gao C, Li C, Huang M (2022) Potential prognostic markers of retained placenta in dairy cows identified by plasma metabolomics coupled with clinical laboratory indicators. Vet Q 42(1):199–212. https://doi.org/10.1080/01652176.2022.2145619

Loiklung C, Sukon P, Thamrongyoswittayakul C (2022) Global prevalence of subclinical ketosis in dairy cows: A systematic review and meta-analysis. Res Vet Sci 144:66–76. https://doi.org/10.1016/j.rvsc.2022.01.003

Luo Z, Zhang y, yin C, yang M, Li J (2023) Application of ARIMA model in infectious disease prediction. In: 2023 5th International Conference on Decision Science and Management (ICDSM), Changsha, China, pp 3–6. https://doi.org/10.1109/ICDSM59373.2023.00012

Magata F, Sone A, Watanabe y, Deguchi y, Aoki T, Haneda S, Ishii M (2021) Prevention of retained fetal membranes and improvement in subsequent fertility with oxytocin administration in cows with assisted calving. Theriogenology 176:200–205. https://doi.org/10.1016/j.theriogenology.2021.09.037

Mahnani A, Sadeghi-Sefidmazgi A, Ansari-Mahyari S, Ghorbani GR, Keshavarzi H (2021) Farm and cow factors and their interactions on the incidence of retained placenta in Holstein dairy cows. Theriogenology 159:87–97. https://doi.org/10.1016/j.theriogenology.2020.10.007

McNeel AK, Reiter BC, Weigel D, Osterstock J, Di Croce FA (2017) Validation of genomic predictions for wellness traits in US Holstein cows. J Dairy Sci 100(11):9115–9124. https://doi.org/10.3168/jds.2016-12323

Mellado M, Herrera CD, De Santiago A, Veliz FG, Mellado J, Garcia JE (2023) Effect of heat stress and body condition score on the occurrence of puerperal disorders in Holstein cows. Span J Agric Res 21(2):e0501. https://doi.org/10.5424/sjar/2023212-19600

Menta PR, Machado VS, Pineiro JM, Thatcher WW, Santos JEP, Vieira-Neto A (2022) Heat stress during the transition period is associated with impaired production, reproduction, and survival in dairy cows. J Dairy Sci 105(5):4474–4489. https://doi.org/10.3168/jds.2021-21185

Molinari PCC, Dahl GE, Sheldon IM, Bromfield JJ (2022) Effect of calving season on metritis incidence and bacterial content of the vagina in dairy cows. Theriogenology 191:67–76. https://doi.org/10.1016/j.theriogenology.2022.08.001

NRC (National Research Council) (2001) Nutrient requirements of dairy cattle, 7th rev edn. National Academy Press, Washington, DC

Overton TR, McArt JAA, Nydam DV (2017) A 100-year review: Metabolic health indicators and management of dairy cattle. J Dairy Sci 100(12):10398–10417. https://doi.org/10.3168/jds.2017-13054

Perez-Baez J, Risco CA, Chebel RC, Gomes GC, Greco LF, Tao S, Thompson IM, Do Amaral BC, Zenobi MG, Martinez N, Staples CR, Dahl GE, Hernandez JA, Santos JEP, Galvao KN (2019) Association of dry matter intake and energy balance prepartum and postpartum with health disorders postpartum: Part II. Ketosis and clinical mastitis. J Dairy Sci 102(10):9151–9164. https://doi.org/10.3168/jds.2018-15879

Perez-Baez J, Silva TV, Risco CA, Chebel RC, Cunha F, De Vries A, Santos JEP, Lima FS, Pinedo P, Schuenemann GM, Bicalho RC, Gilbert RO, Rodriguez-Zas S, Seabury CM, Rosa G, Thatcher WW, Galvao KN (2021) The economic cost of metritis in dairy herds. J Dairy Sci 104(3):3158–3168. https://doi.org/10.3168/jds.2020-19125

Pinedo P, Santos JEP, Chebel RC, Galvao KN, Schuenemann GM, Bicalho RC, Gilbert RO, Rodriguez-Zas S, Seabury CM, Rosa G, Thatcher WW (2020) Early-lactation diseases and fertility in 2 seasons of calving across US dairy herds. J Dairy Sci 103(11):10560–10576. https://doi.org/10.3168/jds.2019-17951

Quintana AR, Sesena S, Garzon A, Arias R (2020) Factors affecting levels of airborne bacteria in dairy farms: A review. Animals 10(3):526. https://doi.org/10.3390/ani10030526

Seyed Almoosavi SMM, Ghoorchi T, Naserian AA, Khanaki C, Drackley JK, Ghaffari MH (2021) Effects of late-gestation heat stress independent of reduced feed intake on colostrum, metabolism at calving, and milk yield in early lactation of dairy cows. J Dairy Sci 104(2):1744–1758. https://doi.org/10.3168/jds.2020-19115

Tao S, Dahl GE (2013) Invited review: Heat stress effects during late gestation on dry cows and their calves. J Dairy Sci 96(7):4079–4093. https://doi.org/10.3168/jds.2012-6278

Vieira-Neto A, Lima FS, Santos JEP, Mingoti RD, Vasconcellos GS, Risco CA, Galvao KN (2016) Vulvovaginal laceration as a risk factor for uterine disease in postpartum dairy cows. J Dairy Sci 99(6):4629–4637. https://doi.org/10.3168/jds.2016-10872

Wessa P (2025) Free Statistics Software, Office for Research Development and Education, version 1.2.1. https://www.wessa.net/

Zeng J, Cai J, Wang D, Liu C, Sun C, Liu J (2023) Heat stress affects dairy cow health status through blood oxygen availability. J Anim Sci Biotechnol 14(1):112. https://doi.org/10.1186/s40104-023-00915-3

Published
2025-12-30
How to Cite
Pérez-Rebolloso, E., García, J. E., Morales, J. L., Calderón, M. G., Alvarado, A. S., Macías-Cruz, U., Avendaño-Reyes, L., Mellado, J., & Mellado, M. (2025). Time series modeling of retained placenta, metritis, and ketosis in Holstein cows and heifers and its association with climate variables in a hot-arid zone. Agricultural Science and Practice, 12(3), 27-37. https://doi.org/10.15407/agrisp12.03.027