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https://doi.org/10.15407/agrisp5.03.054
VIABILITY OF SPERM CELLS OF BOARS AT THE ADDITION OF FINELY DISPERSIVE SILICA TO CRYOPRESERVATION AND DEFROSTING MEDIA
O. V. Shcherbak, S. I. Kovtun

Institute of Animal Breeding and Genetics n.a. M. V. Zubets, NAAS of Ukraine, off. 208, Pogrebniak Str, Chubynske village, Boryspil district, Kyiv region, 08321, Ukraine

E-mail: ov19792006@gmail.ua

Received September 09, 2018 / Received October 08, 2018 / Accepted November 21, 2018
Abstract
Aim. In modern conditions the priority task of preserving biological diversity is increasing the role of agricul- ture in its maintenance. Within the system of long-term preservation of genetic resources of farm livestock, urgent improvement is needed in the fi eld of media for dilution, cryopreservation and storage of genetic mate- rial of animals; technological elements of cryopreservation of genetic material of animals and biotechnologi- cal means of obtaining embryos from cryopreserved genetic material outside of the organism. Methods. It is known that the medium for boar sperm cryopreservation is added the following components: hydrophilic extract of oaken silkworm pupa, aqueous extract of propolis, bovine serum albumin, water-soluble components of yolk and lipoproteins, extract of crude sunfl ower oil, proline, trimethylglycine (betaine) and nanomaterials. Results. The results of experimental studies on the interaction between cryopreserved ejaculated sperm cells of boars with nanoparticles of fi nely dispersive silica (FDS) and saccharose were presented. It is noteworthy that the studies are related both to the technology of gamete cryopreservation and its de-preservation. Nano- material А-300 with S spec = 285 sq.m./g (Kalush, Ukraine) was used in the studies after previous heating for 2 h at 200°С and surface modifi cation with the carbohydrate – saccharose. FDS/saccharose was added to the cultivation medium for defrosted sperm cells (2.9 % sodium citrate solution) as well as prior to freezing into lactose-yolk-glycerin cryomedium in the concentrations of 0.1; 0.01 and 0.001 %. The analysis of obtained results demonstrated different impact of nanomaterial, used by us as an additive to media. It was established that after defrosting, boar sperm cells with FDS/saccharose in 0.001 % concentration demonstrated the acti- vity at the level of 14.2 % and the total time of their survival was 4.0 h. FDS/saccharose concentration of 0.001 %, applied during the cryopreservation of ejaculated boar sperm, ensured 25.0 % activity after defrost- ing with the total survival time of 5.5 h. Conclusions. The article refl ects the promising nature of conducting further biotechnological studies with the application of nanomaterials of different origin in the system of pres- ervation and rational use of genetic resources of farm livestock.
Key words: ejaculated sperm cells, boar, nanomaterial, fi nely dispersive silica, cryopreservation, gene fund preservation.
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