VIABILITY OF SPERM CELLS OF BOARS AT THE ADDITION
OF FINELY DISPERSIVE SILICA TO CRYOPRESERVATION
AND DEFROSTING MEDIA
O. V. Shcherbak, S. I. Kovtun
Institute of Animal Breeding and Genetics n.a. M. V. Zubets, NAAS of Ukraine,
off. 208, Pogrebniak Str, Chubynske village, Boryspil district, Kyiv region, 08321, Ukraine
Received September 09, 2018 / Received October 08, 2018 / Accepted November 21, 2018
Aim. In modern conditions the priority task of preserving biological diversity is increasing the
role of agricul-
ture in its maintenance. Within the system of long-term preservation of genetic resources of farm
urgent improvement is needed in the fi eld of media for dilution, cryopreservation and storage of
rial of animals; technological elements of cryopreservation of genetic material of animals and
cal means of obtaining embryos from cryopreserved genetic material outside of the organism.
is known that the medium for boar sperm cryopreservation is added the following components:
extract of oaken silkworm pupa, aqueous extract of propolis, bovine serum albumin, water-soluble
of yolk and lipoproteins, extract of crude sunfl ower oil, proline, trimethylglycine (betaine) and
Results. The results of experimental studies on the interaction between cryopreserved ejaculated
of boars with nanoparticles of fi nely dispersive silica (FDS) and saccharose were presented. It is
that the studies are related both to the technology of gamete cryopreservation and its
material А-300 with S spec = 285 sq.m./g (Kalush, Ukraine) was used in the studies after previous
2 h at 200°С and surface modifi cation with the carbohydrate – saccharose. FDS/saccharose was added
cultivation medium for defrosted sperm cells (2.9 % sodium citrate solution) as well as prior to
lactose-yolk-glycerin cryomedium in the concentrations of 0.1; 0.01 and 0.001 %. The analysis of
results demonstrated different impact of nanomaterial, used by us as an additive to media. It was
that after defrosting, boar sperm cells with FDS/saccharose in 0.001 % concentration demonstrated
vity at the level of 14.2 % and the total time of their survival was 4.0 h. FDS/saccharose
0.001 %, applied during the cryopreservation of ejaculated boar sperm, ensured 25.0 % activity
ing with the total survival time of 5.5 h. Conclusions. The article refl ects the promising nature
further biotechnological studies with the application of nanomaterials of different origin in the
system of pres-
ervation and rational use of genetic resources of farm livestock.
ejaculated sperm cells, boar, nanomaterial, fi nely dispersive
silica, cryopreservation, gene fund
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