TY - JOUR AU - Biloivan, O. V. AU - Stegniy, B. T. AU - Gerilovych, A. P. AU - Arefiev, V. L. AU - Wölfel, R. AU - Schwarz, J. AU - Popp, C. AU - Grass, G. PY - 2020/12/25 Y2 - 2024/03/29 TI - Screening of possibly anthrax-contaminated burial sites in eastern and southern Ukraine JF - Agricultural Science and Practice JA - Agric. sci. pract. VL - 7 IS - 3 SE - Articles DO - 10.15407/agrisp7.03.003 UR - https://agrisp.com/index.php/agrisp/article/view/2020_03_01 SP - 3-14 AB - Aim. The aim of this study was to screen soil samples of 17 anthrax burial sites in Eastern and Southern Ukrainefor the presence of B. anthracis. Methods. Soil samples were collected from anthrax grave sites located in Kharkiv,Sumy and Mykolaiv regions (diseased animals dated from 1946 to 2003). Isolation of B. anthracis from collected soilsamples was performed with the GABRI method. From single colonies without hemolysis, that were inactivated withperacetic acid- containing 2 % Terralin PAA solution, DNA was extracted and analyzed by qPCR for the presence ofchromosomal marker dhp61, as well as the markers pagA and capC located on virulence plasmids pXO1 and pXO2,respectively. Results. Eleven fi eld trips were conducted from July, 2016 to October, 2018 in which 369 soil samplesfrom 17 burial sites in Kharkiv, Sumy and Mykolaiv oblasts were collected from different depths of presumed anthraxcarcass sites. In most cases (12 out of 17 cases), the current status of these burial sites was deteriorated and not prop-erly accounted for. It was possible to obrain viable B. anthracis isolate was obtained from 50 cm depth at the gravesite near Koviagy village, Valky district, Kharkiv region (49.92373°N, 35.48951°E). This isolate was named KhR/VD/Kov2-2-05-3 and deposited in the Collection of Animal Infectious Pathogens of the National Scientifi c Center“Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine. The contamination level of soil atthe isolation site reached about 10 4 CFU per g as determined by plate counting. qPCR analysis of this isolate identi-fi ed both the dhp61 B. anthracis chromosomal and the pagA virulence plasmid marker. However, the plasmid pXO2marker, required for capsule-formation could not be detected. Conclusions. The anthrax burial sites were createdbetween the 1920s and 1960s, however, only approximate locations could be found and demarcated. In most cases thestatus of the sites was unsuitable for sampling. Nevertheless, isolation of B. anthracis in one case in the Valky districtshows that old anthrax burial sites (13.500 exist in Ukraine) still pose a risk as potential source of the infection andtherefore require more attention and surveillance, for which a surveillance plan will be developed. ER -