online ISSN:2312-3389 print ISSN:2312-3370 DOI:10.15407/agrisp

Archive

Archive of Agricultural Science and Practice Journal issues

List of all issues / Content of issue 2019-1 / Abstract & References of Article 6
https://doi.org/10.15407/agrisp6.01.060
CHROMOSOMAL ANOMALIES IN DAIRY CATTLE AS REASONS OF IMPAIRED FERTILITY
V. V. Dzitsiuk, H. T. Tipilo

Institute of Animal Breeding and Genetics NAAS, named after M.V. Zubets 1, Pohrebniaka Str., Chubynske village, Boryspil District, Kyiv Region, Ukraine 08321

E-mail: valentynadzitsiuk@gmail.com

Received January 10, 2019 / Received February 15, 2019 / Accepted March 22 , 2019
Abstract
Aim. The evaluation of animals for the presence of chromosomal anomalies is one of the main tasks of prac- tical selection, aimed at detecting undesired chromosomal anomalies in early age, which may have negative impact on the reproductive and productive capability of cows and lead to considerable economic losses. The aim of the work is a cytogenetic analysis of the chromosome set in cows of Ukrainian Red-and-Motley dairy cattle breed, which will allow assuming a decrease in reproductive functions with chromosomal aberrations. Methods. We examined 53 cows of the Ukrainian Red-and-Motley dairy cattle breed in SE Research Farm Khrystynivske, IABG named after M.V. Zubets, NAAS. The investigation of chromosomal anomalies involved 72-h cultivation of lymphocytes from the peripheral blood of animals using the common methods. During a routine analysis the preparations were stained with 2 % Giemsa staining solution. The induction of G-bands for differential staining of chromosomes was conducted using 0.25 % solution of trypsin. The processing of study results was performed with Microsoft Excel software package. Results. The investigations in the aberration spectrum detected aneuploid and polyploid cells, breaks and fragments of chromosomes, premature chromo- some disjunction in mitosis and translocation. The total number of aberrant cells in cows with decreased fertil- ity was 14.69 ± 0.56 %, the number of aberrations per one investigated cell was 0.144, which was almost twice reliably (Р < 0.999) exceeding the values of similar features for cows which did not have problems with repro- duction. GTG-banding method was used to detect a new RT 13/23 Robertsonian translocation. Conclusions. The cytogenetic analysis of chromosome set of Ukrainian Red-and-Motley dairy breed cows allows assuming the connection between a decrease in the fertility of cows and chromosomal instability. A routine screening of dairy cows allows both evaluating the karyotype saturation with undesired chromosomal aberrations and using the obtained results to forecast the reproductive ability of an animal in the early age.
Key words: karyotype, aberrations, Ukrainian Red-and-Motley breed, reproductive ability.
References

1. Yimer N. Chromosomal Anomalies and Infertility in Farm Animals: A review. Pertanika J. Tropical Agric. Sci. 2014;37(1):1-18.
 
2. Gustavsson I. Chromosome Abnormality in Cattle. Nature. 1966;211:865-6.
https://doi.org/10.1038/211865a0
 
3. Terje Raudsepp, Bhanu P Chowdhary. Chromosome Aberrations and Fertility Disorders in Domestic Animals. Ann. Rev. Anim. Bioscie. 2016;4:15-43.
https://doi.org/10.1146/annurev-animal-021815-111239
 
4. De Lorenzi L, Morando P, Planas J, Zannotti M, Molte- ni L, Parma P. Reciprocal translocations in cattle: frequency estimation. J. Anim. Breed. Genet. 2012;129(5):409-6.
https://doi.org/10.1111/j.1439-0388.2011.00983.x
 
5. Khatun MR, Arifuzzaman M, Ashraf A. Karyotype for Identifi cation of Genetic Abnormalities in Cattle. Asian J. Anim. Veterin. Advan. 2011;6(2):117-25.
https://doi.org/10.3923/ajava.2011.117.125
 
6. Szczerbal I, Switonski M. Chromosome Abnormalities in Domestic Animals as Causes of Disorders of Sex Development or Impaired Fertility. Insights Anim. Rep- rod. 2016;9.
https://doi.org/10.5772/62053
 
7. Trukhachev VI, Oleynik SA, Zlydnev NZ, Morozov VJu, Selionova MI, Chizhova LN, Skokova AV. Cha- acteristics of the chromosome set of Holstein cows with reproductive disorders in the North-Caucasian cattle population. Cytol. Genet. 2017;51(4):272-7.
https://doi.org/10.3103/S0095452717040119
 
8. EL-Bayomi KM, EL-Araby IE.-S, Zaglool AW. Cytogenetic analysis related to some infertility problems in Cattle. Global Veterinaria. 2011;7(4):323-9.
 
9. Switonski M. 50 years of studies on bovine 1/29 Ro- bertsonian translocation-from Giemsa staining to ge- nomic analysis. Conference Paper in Chromosome Re- search, Ischia, Naples, Italy. 2014;22:395.
 
10. Anaya G, Valera M, Moreno-Millán M, Azor P, Peral- García P, Demyda-Peyrás S. Sex chromosomal abnor- malities associated with equine infertility: validation of a simple molecular screening tool in the Purebred Spa- nish Horse. Anim. Genet. 2017;48(4):404-11.
https://doi.org/10.1111/age.12543
 
11. Udroiu I, Sgura A. Cytogenetic tests for animal pro- duction: state of the art and perspectives. Anim. Genet. 2017;48(5):505-15.
https://doi.org/10.1111/age.12581
 
12. De Lorenzi L, Genualdo V, Gimelli S, Rossi E, Perucat- ti A, Iannuzzi A, Zannotti M, Malagutti L, Molteni L, Iannuzzi L, Parma P. Genomic analysis of cattle rob(1;29). Chromosome Res. 2012:20(7):815-23.
https://doi.org/10.1007/s10577-012-9315-y
 
13. Yimer N, Rosnina Y. Chromosomal anomalies and in- fertility in farm animals: a review. Pertanika J. Trop. Agric. Sci. 2014;37(1):1-18.
 
14. De Lorenzi L, Rossi E, Gimelli S, Parma P. De no- vo reciprocal translocation t(5;6)(q13;q34) in cattle: cytogenetic and molecular characterization. Cytogenet Genome Res. 2014;142(2):95-100.
https://doi.org/10.1159/000356209
 
15. Ciptadi Gatot, Nur Ihsan, Muhammad-Rahayu, Sri- Nurgiartiningsih V-Mudawamah, Mudawamah-Putri Ir- santi. The Comparison of Chromosome Analysis Re- sult by Manual and Software Cytovision Image Ana- lysis Using Simple G-Banding Research. J. Life Sci. 2017;4(2);106-10.
https://doi.org/10.21776/ub.rjls.2017.004.02.3
 
16. Dumont BL. Variation and evolution of the meiotic requirement for crossing over in mammals. Genetics. 2017;205(1):155-68.
https://doi.org/10.1534/genetics.116.192690
 
17. Rodero-Serrano E, Demyda-Peyrás S, González-Mar- tineza A, Rodero-Franganillo A, Moreno-Millán V. The rob(1;29) chromosome translocation in endangered Andalusian cattle breeds. Livestock Sci. 2013;158(1-3): 32-9.
https://doi.org/10.1016/j.livsci.2013.10.001
 
18. Omaima H Ezzo, Farghaly AA, Amal M Abo El-Ma- aty, Karima Gh MM. Hormonal and Cytogenetic In- vestigations in Mares with Early Embryonic Death. Global Veterin. 2011;7(3):211-8.
 
19. O'Connor RE, Fonseka G, Frodsham R, Archibald AL, Lawrie M, Walling GA, Griffi n DK. Isolation of sub- telomeric sequences of porcine chromosomes for trans- location screening reveals errors in the pig genome assembly. Anim. Genet. 2017;48(4):395-403.
https://doi.org/10.1111/age.12548