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https://doi.org/10.15407/agrisp6.01.010
POLYMORPHISM OF BUCKWHEAT SEED STORAGE PROTEINS IN CULTIVAR GROUPS, DIFFERING BY THEIR MORPHOTYPE
Ye. V. Zaika1 , N. A. Kozub2, 3 , I. A. Sozinov2 , G. Ya. Bidnyk2, 3 , P. P. Karazhbey1

1NSC “Institute of Agriculture” NAAS, 2b, Mashynobudivnykiv Str., Chabany village, Kyiv-Sviatoshyn District, 08163
2 Institute of Plant Protection, NAAS, 33, Vasylkivska Str., Kyiv 03022
3 Institute of Food Biotechnology and Genomics, NAS of Ukraine 2a, Osypovskoho Str., Kyiv 04123

E-mail: za-ika@ukr.net

Received December 26, 2018 / Received February 26, 2019 / Accepted March 22, 2019
Abstract
Aim. To study polymorphism of buckwheat seed proteins in cultivar groups of different morphotypes by the color of corolla. Methods. Electrophoresis by Laemmli method in 17.5 % separating PAAG gel. Results. Electrophoresis spectrum variants with the incidence from 1 to 76 %, common for buckwheat cultivar groups, which are different in their morphotype, were revealed. The obtained results demonstrate a particular level of heterogeneity by the revealed variants in each investigated group of buckwheat genotypes. Variants 2h and 4f, occurring with the incidence of 10 % and 8 % respectively, and specifi c for green-fl owered group of buckwheat cultivars, were also revealed. Conclusions. Different incidence of electrophoresis spectrum components in buckwheat morphotypes, different in fl ower coloring, demonstrates probable different selective value of genes, which control the synthesis of these components or their relation to the genes, closely bound to them. Taking this fact into consideration, buckwheat seed proteins may be molecular and genetic markers for identifi cation of some buckwheat morphotypes, which may be used in breeding programs.
Key words: edible buckwheat, storage proteins, globulins, albumins, polymorphism.
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